ros fluorometric assay kit  (Elabscience Biotechnology)

Journal: MedComm

Article Title: Ipriflavone From Aquilaria malaccensis Lam. Exosome‐Like Nanoparticles Targets Prolyl Hydroxylase Domain Protein 2 (PHD2) to Enhance Hypoxia‐Inducible Factor‐α (HIF‐α) Hydroxylation Thereby Alleviating Hypoxia‐Induced Gastrointestinal Mucosal Ferroptosis

doi: 10.1002/mco2.70722

Figure Lengend Snippet: Oral administration of AELNs ameliorated gastric and small intestinal mucosal ferroptosis caused by hypoxia in mice. The mice were housed in a vented hypoxic chamber for 3 days ( n = 10 mice in each group). They received PBS or AELNs (1 × 10 5 particles) via oral gavage on Day 1 and were euthanized on Day 3. Representative photographs showing the microstructures of the murine gastric (A) and small intestinal mucosal cells (B) under transmission electron microscopy (TEM). The red arrows indicate shrunken mitochondria (scale bar: overview, 1 µm; inset, 500 nm). Quantification of Fe 2+ , reactive oxygen species (ROS), lipid peroxidation (LPO), and 4‐hydroxynonenal (4‐HNE) of the gastric (C) and small intestinal (D) mucosa ( n = 10). Western blot analysis of the indicated protein levels in the gastric (E) and small intestinal (F) mucosa assessed by Western blot. (G) Cytokines in the gastric (upper) and small intestinal (lower) mucosa ( n = 10). Error bars indicate mean ± SEM. One‐way ANOVA: * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001; ns, not significant.

Article Snippet: ROS, 4‐HNE, and LPO levels were measured using kits from Elabscience (ROS, E‐BC‐K138‐F; 4‐HNE, E‐EL‐0128c; LPO, E‐BC‐K176‐M; Wuhan, Hubei, China).

Techniques: Transmission Assay, Electron Microscopy, Western Blot

Journal: Current Issues in Molecular Biology

Article Title: Protective Effect of Apocynum venetum L. Leaves Extract Against Diabetic Cardiomyopathy: Inhibition of Oxidative Stress and Ferroptosis via Modulation of the Xc − /GSH/GPX4 Axis

doi: 10.3390/cimb48040375

Figure Lengend Snippet: AVLE inhibits ferroptosis in HGHF-induced H9c2 cells. Contents of ( A ) Fe 2+ in H9C2 cells. ( B1 ) Representative images and ( B ) fluorescence intensity of DCFH-DA. ( C1 ) Representative images and ( C ) JC-1 fluorescence ratio reflecting MMP. ( D1 ) The images and ( D ) fluorescence intensity of FerroOrange (scale bar = 100 μm); ( E ) representative blot images and the expression levels of GPX4 ( E1 ), FTH-1 ( E2 ), SLC7A11 ( E3 ), TfR1 ( E4 ) and ACSL4 ( E5 ) in H9c2 cells. Data are reported as mean ± SEM (n = 3, ## p < 0.01, ### p < 0.001 vs. CON; * p < 0.05, ** p < 0.01, *** p < 0.001 vs. HGHF).

Article Snippet: Superoxide production, mitochondrial membrane potential (MMP), and ferrous ion (Fe 2+ ) levels were detected using the DCFH-DA (E-BC-K138-F, Elabscience, Wuhan, China), JC-1 (C2006, Beyotime, Shanghai, China), and FerroOrange (F374, Dojindo, Kumamoto, Japan) fluorescent probes, respectively.

Techniques: Fluorescence, Expressing